A buffer solution resists changes in pH by containing both a weak acid and its conjugate base.
A buffer solution resists changes in pH by containing both a weak acid and its conjugate base. The Henderson-Hasselbalch equation lets you calculate the buffer's pH directly from the acid's pKa and the ratio of base to acid present.
A buffer uses acetic acid (pKa = 4.76) with [A-] = 0.2 M and [HA] = 0.1 M. pH = 4.76 + log₁₀(0.2/0.1) = 4.76 + 0.301 ≈ 5.06.
It calculates the pH of a buffer solution based on the ratio of a weak acid to its conjugate base.
pH = pKa + log10([conjugate base] ÷ [weak acid]).
Buffers resist changes in pH when small amounts of acid or base are added, which is essential for maintaining stable conditions in biological systems like blood and in many chemical processes.